Real-Time PCR Detection of Parapoxvirus DNA,
نویسندگان
چکیده
منابع مشابه
Real-time PCR detection of parapoxvirus DNA.
BACKGROUND Detection of parapoxviruses is important in various animals as well as in humans as zoonotic infections. Reliable detection of parapoxviruses is fundamental for the exclusion of other rash-causing illnesses, for both veterinarians and medical practitioners. To date, however, no real-time PCR assay for the detection of parapoxviruses has been reported. METHODS A minor groove binder-...
متن کاملRuminant DNA detection: Real-time PCR Detection of Ruminant DNA
In order to control the spread of transmissible spongiform encephalopathy (TSE), several DNA methods have been described for the detection of the species origin of meat-andbone-meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semi-quantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of ...
متن کاملApplication of Rapid and Sensitive Real Time PCR Technique in Detection of DNA Impurities in Recombinant Interferon
Background & Objective: Interferon belongs to a family of cytokines, which has the most important role in the innate immune response to virus infections. While producing recombinant interferon in biological host, some pieces of host nucleic acids remain in product. Because of limitations in previous techniques for detection of these impurities, the objective of this study is to use rapid ...
متن کاملA rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...
متن کاملQuantitative detection of Tropheryma whipplei DNA by real-time PCR.
Over the past decade, PCR-based methodologies have been introduced to complement or even replace histopathologic study of biopsy specimens for the diagnosis of Whipple’s disease (12). However, positive PCR results have been reported on testing small-bowel and saliva specimens from asymptomatic patients (3, 4, 11). Although these results have not been independently confirmed (8), they have nonet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2006
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2005.060335